Synthesis of ribonuclease-resistant ribonucleic acid by fowl-plague-virus-induced ribonucleic acid polymerase
نویسندگان
چکیده
منابع مشابه
Ribonucleic Acid and protein synthesis in chick embryo cells infected with fowl plague virus.
Fowl plague virus comprised four major protein components and several minor ones, two strains of the virus giving similar results. One of the components was identified as the nucleocapsid protein. Synthesis of the virion proteins could readily be detected in infected cells 3 hr after infection. The two subcellular fractions associated with viral ribonucleic acid (RNA) polymerase activity (nucle...
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A ribonucleic acid (RNA)-dependent RNA polymerase was induced in chick embryo fibroblast cells after infection with Sendai virus (parainfluenza 1 virus). The enzyme was associated with the microsomal fraction of infected cells and reached maximum detectable activity at 18 hr after virus infection. The activity of the enzyme in vitro was dependent on the presence of added magnesium ions and all ...
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The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
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Numerous reports have been published on the properties and reaction mechanism of the highly purified enzyme preparations that catalyze the deoxyribonucleic acid-directed synthesis of ribonucleic acid (l-6). The various RNA polymerases tudied, although differing in some respects, show a remarkable similarity in their cofactor requirements, reaction kinetics, physical properties, and facility to ...
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The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1969
ISSN: 0306-3283
DOI: 10.1042/bj1140064pa